sox2 antibody - bsa free  (Bio-Techne corporation)

Journal: Cells

Article Title: Rat Plantar Fascia Stem/Progenitor Cells Showed Lower Expression of Ligament Markers and Higher Pro-Inflammatory Cytokines after Intensive Mechanical Loading or Interleukin-1β Treatment In Vitro.

doi: 10.3390/cells12182222

Figure Lengend Snippet: Figure 5. Expression of stemness markers in PFCs compared to BMSCs. (A) Photomicrographs showing the immunocytochemical staining of Nanog, Oct4, Sox2, and nucleostemin (NS) (upper left: primary antibody, lower left: DAPI, right: merged). Magnification: 200×; scale bar: 100 µm; n = 3/group. (B) Bar charts showing the mRNA expression of Nanog, Oct4, and Sox2 in PFCs and BMSCs. *** p < 0.001; n = 6/group.

Article Snippet: The cells were then washed with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.25% Triton X-100 for 10 min, and blocked with 5% normal goat serum for 1 h. Afterwards, the cells were incubated with mouse anti-rat Ki67 (ab279653), rabbit anti-rat Nucleostemin (NS) (ab70346) (both from Abcam, Cambridge, UK), rabbit anti-rat Nanog (NBP1-77109), Oct4 (NB100-2379SS) or Sox2 (NB110-37235SS) (all from Novus Biologicals, Centennial, CO, USA) (1:200 all) overnight at 4 ◦C.

Techniques: Expressing, Staining

Journal: iScience

Article Title: WNT1-inducible signaling pathway protein 1 activation through C-X-C motif chemokine ligand 5/C-X-C chemokine receptor type 2/leukemia inhibitory factor/leukemia inhibitory factor receptor signaling promotes immunosuppression and neuroendocrine differentiation in prostate cancer

doi: 10.1016/j.isci.2024.110562

Figure Lengend Snippet:

Article Snippet: SOX2 , NOVUS , NB110-37235.

Techniques: Recombinant, Transfection, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software, ChIP-sequencing

Journal: Neural Regeneration Research

Article Title: Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma

doi: 10.4103/1673-5374.353848

Figure Lengend Snippet: Müller cell regeneration and gliosis occur after blunt ocular trauma. (A) Representative images of Ki67 (green) and GS (red) staining. After blunt ocular trauma, the number of Ki67/GS double-positive cells increased over time and reached a peak on the third day. (B) The number of Ki67/GS double-positive cells was recorded. (C) Representative images of SOX2 (green) and GS (red) staining. The number of SOX2/GS double-positive cells increased over time after blunt ocular trauma. (D) The number of SOX2/GS double-positive cells was recorded. (E) Representative images of GFAP (green) staining. The number of GFAP-positive cells increased on the seventh day after blunt ocular trauma. Scale bar: 50 μm. (F) The number of GFAP-positive cells was recorded. Data are shown as the mean ± SEM ( n = 5 per group). * P < 0.05, vs . control group (Student’s t -test). GCL: Ganglion cell layer; GFAP: glial fibrillary acidic protein; GS: glutamine synthetase; INL: inner nuclear layer; ONL: outer nuclear layer; SOX2: SRY-box transcription factor 2.

Article Snippet: Sections were then incubated at 4°C overnight with one or more of the following primary antibodies [all diluted 1:100 by a fresh blocking buffer (0.01 M PBS containing 0.3% Triton X-100 and 3% bovine serum albumin)]: synaptophysin (Syn; rabbit; Aviva Systems Biology, San Diego, CA, USA; Cat# ARP45435, RRID: AB_2048301), Ki67 (rabbit; Proteintech, Wuhan, China; Cat# 27309-1-AP, RRID: AB_2756525), glutamine synthetase (GS; mouse; Proteintech; Cat# 66323-2-Ig, RRID: AB_2881704), CD45 (rabbit; Proteintech; Cat# 20103-1-AP, RRID: AB_2716813), sex determining region Y-box 2 (SOX2) (rabbit; Novus, Littleton, CO, USA; Cat# NB110-37235, RRID: AB_792070), protein kinase C (PKC; mouse; Enzo, New York, NY, USA; Cat# ADI-KAM-PK020, RRID: AB_10998587), inducible nitric oxide synthase 2 (iNOS) (mouse; Santa Cruz Biotechnology, Dallas, TX, USA; Cat# sc-7271, RRID: AB_2891105), glial fibrillary acidic protein (GFAP; mouse; Millipore, Burlington, MA, USA; Cat# MAB3402, RRID: AB_94844), calretinin (rabbit; Abcam, Cambridge, UK; Cat# ab702, RRID: AB_305702), choline acetyltransferase (ChAT; rabbit; Abcam; Cat# ab6168, RRID: AB_2244866), ionized calcium binding adapter molecule 1 (Iba1) (rabbit; Abcam; Cat# ab178847, RRID: AB_2832244), Iba1 (mouse; Abcam; Cat# ab15690, RRID: AB_2224403), calbindin-D (rabbit; Sigma, St. Louis, MO, USA; Cat# c2724, RRID: AB_258818), or CD16/32 (rabbit; Abcam; Cat# ab223200).

Techniques: Staining, Control